Sims 2008 protocol

The intracellular distribution of glycolytic and other enzymes in rat-brain homogenates and mitochondrial preparations. Download references. You can also search for this author in PubMed Google Scholar. Correspondence to Neil R Sims. Reprints and Permissions. Isolation of mitochondria from rat brain using Percoll density gradient centrifugation.

Nat Protoc 3, — Download citation. Published : 03 July Issue Date : July Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article.

Provided by the Springer Nature SharedIt content-sharing initiative. Cellular and Molecular Neurobiology By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Advanced search. Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily. Skip to main content Thank you for visiting nature. Abstract We have developed procedures that combine differential centrifugation and discontinuous Percoll density gradient centrifugation to isolate mitochondria from rat forebrains and brain subregions.

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Figure 1. Figure 2: Typical appearance of a centrifuge tube at the conclusion of the density gradient centrifugation step in Method A Step 12A ix. References 1 Schneider, W. Google Scholar 4 Clark, J. Google Scholar 5 Stahl, W. Article Google Scholar 23 Oliveira, J. View author publications.

Rights and permissions Reprints and Permissions. About this article Cite this article Sims, N. Copy to clipboard. Petrov Irina A. Redell Pramod K. Dash Scientific Reports Comments By submitting a comment you agree to abide by our Terms and Community Guidelines. Search Search articles by subject, keyword or author. Show results from All journals This journal.

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